Photosynthetic Electron Transport in an Anoxygenic Photosynthetic Bacterium Afifella (Rhodopseudomonas) marina Measured Using PAM Fluorometry.

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Raymond J. Ritchie and John W. Runcie

Research paper in Photochemistry and Photobiology, doi: 10.1111/j.1751-1097.2012.01241.x. “Photosynthetic Electron Transport in an Anoxygenic Photosynthetic Bacterium Afifella (Rhodopseudomonas) marina Measured Using PAM Fluorometry.”

This paper describes the use of the Blue-RAT in determining values of photosynthetic absorptance, a requirement for the calculation of Electron Transport Rate (ETR).  As Afifella marina is a unicellular organism (a purple nonsulphur anoxygenic photobacterium), cells were filtered onto glass fibre filters prior to absorptance measurements.  Implications of the research include a more accurate method for calculating ETR of unicellular algae, and a means of assessing the rates of H2 production and nitrogen fixation by anoxygenic photobacteria (both clearly important commercial activities).

A PDF of the paper can be accessed here:

Photochem Photobiol. 2013 Mar-Apr;89(2):370-83.